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Objective:To investigate the protein and messenger RNA expression of glucose transporter 4 in endometrium of women with polycystic ovary syndrome(PCOS),and to evaluate its change after three months treatment of metformin.Methods:Twenty-two patients with polycystic ovary syndrome(group A)and six non-PCOS infertile women(group B)were recruited in our hospital.The consent form was obtained from each patient.Endometrium and blood samples were obtained during the proliferative phase of the menstrual cycle.Reverse transcriptase-polymerase chain reaction(RT-PCR)and immunohistochemical method were applied to detect the expression of glucose transporter 4(GLUT4)in endometrium.All PCOS patients received monotherapy of metformin after endometrium biopsy.Seven un-conceived patients(group A1)from group A who completed three months of metformin treatment were selected to perform the second time biopsy during proliferative phase.The expression of GLUT4 were remeasured as well.Results:There were no significant differences of the levels of E2,P and endometrium thickness on the biopsy day between group A and group B.But the basal levels of LH,T,LH/FSH ratio,and the ovarian volume were significantly higher in group A as compared with group B(P<0.001).The expression of GLUT4 in group A was significantly lower than that of group B(1.05±0.13 vs 1.50±0.21,P<0.001).In group A1,the expression of GLUT4 in endometrium were changed from 1.08±0.08 to 1.27±0.16 before and after treatment(P<0.05).The results of immunohistochemical staining of GLUT4 in endometrium were coincident with the results of RT-PCR.The fast insulin level and the insulin sensitivity index were also improved obviously after three months of metformin therapy(P<0.05).Conclusions:Insulin-resistance status was proved existing in endometrium of PCOS women;A significant improvement of GLUT4 expression was observed in endometrium after metformin treatment,supporting that metformin can relief insulin-resistant status of the endometrium in PCOS patients.
Abstract：Objective:To investigate the protein and messenger RNA expression of glucose transporter 4 in endometrium of women with polycystic ovary syndrome(PCOS),and to evaluate its change after three months treatment of metformin.Methods:Twenty-two patients with polycystic ovary syndrome(group A)and six non-PCOS infertile women(group B)were recruited in our hospital.The consent form was obtained from each patient.Endometrium and blood samples were obtained during the proliferative phase of the menstrual cycle.Reverse transcriptase-polymerase chain reaction(RT-PCR)and immunohistochemical method were applied to detect the expression of glucose transporter 4(GLUT4)in endometrium.All PCOS patients received monotherapy of metformin after endometrium biopsy.Seven un-conceived patients(group A1)from group A who completed three months of metformin treatment were selected to perform the second time biopsy during proliferative phase.The expression of GLUT4 were remeasured as well.Results:There were no significant differences of the levels of E2,P and endometrium thickness on the biopsy day between group A and group B.But the basal levels of LH,T,LH/FSH ratio,and the ovarian volume were significantly higher in group A as compared with group B(P<0.001).The expression of GLUT4 in group A was significantly lower than that of group B(1.05±0.13 vs 1.50±0.21,P<0.001).In group A1,the expression of GLUT4 in endometrium were changed from 1.08±0.08 to 1.27±0.16 before and after treatment(P<0.05).The results of immunohistochemical staining of GLUT4 in endometrium were coincident with the results of RT-PCR.The fast insulin level and the insulin sensitivity index were also improved obviously after three months of metformin therapy(P<0.05).Conclusions:Insulin-resistance status was proved existing in endometrium of PCOS women;A significant improvement of GLUT4 expression was observed in endometrium after metformin treatment,supporting that metformin can relief insulin-resistant status of the endometrium in PCOS patients.
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中图分类号:R711.75
引用信息:
[1]林仙华,叶碧绿,徐炳森,等.The expression of glucose transporter 4 in endometrium of polycystic ovary syndrome women and its change after metformin treatment[J].生殖医学杂志,2007(S1):39-45.
2007-12-15
2007-12-15