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目的探讨吸烟对正常男性和弱精子症男性精子DNA完整性的影响。方法采用病例-对照配对法,通过染色质扩散法测定了重度吸烟和从不吸烟的164例正常男性和120例弱精子症男性的精子DNA碎片指数(DFI)。结果重度吸烟者相比非吸烟者DFI显著升高(P<0.01),其值分别为17.8和14.6。弱精子症患者与正常男性相比DFI显著升高(P<0.001),其值分别为19.2和14.9。在正常精子男性中,重度吸烟者比非吸烟者DFI显著升高(P<0.05),而在弱精子症患者中,重度吸烟者也比非吸烟者的DFI显著升高(P<0.05)。结论弱精子症患者比正常男性的DNA完整性降低。重度吸烟对于正常男性和弱精子症患者都是一个损伤DNA的危险因子。吸烟可能与其它病理因素共同影响精子的DNA完整性。
Abstract：Objective:To investigate the impact of smoking on sperm DNA integrity in patients with normozoospermic and asthenozoospermic. Methods:This paired case-control study was conducted in heavy-smoking or non-smoking patients including 164 normozoospermic and 120 asthenozoospermic patients.Sperm DNA integrity presented by the DNA fragmentation index(DFI)was assayed by sperm chromatin dispersion(SCD)method. Results:The DFI of heavy smokers was significantly higher than that of non-smokers(P<0.001), with values of 17.8 and 14.6 respectively.The asthenozoospermic patients had higher DFI compared to the normozoospermic patients(P<0.001),with values of 19.2 and 14.9 respectively.The heavy-smoking normozoospermic patients had significantly higher DFI than the non-smoking normozoospermic patients (P<0.05).The heavy-smoking asthenozoospermic patients also had significantly higher DFI than non-smoking asthenozoospermic patients(P<0.05). Conclusions:Asthenozoospermic patients have lower sperm DNA integrity than normozoospermic patients.Heavy-smoking can impair sperm DNA integrity in both normozoospermic and astheznozoospermic patients.Smoking is a risk factor for sperm DNA integrity.
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基本信息:
中图分类号:R698.2
引用信息:
[1]余波澜,周宇林,陈康,等.吸烟对正常男性和弱精子症男性精子DNA完整性的影响[J].生殖医学杂志,2013,22(06):404-408.
基金信息:
广州市科技计划项目(2012J4300089);; 广州医学院基金(2011C09)资助
2013-06-15
2013-06-15