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目的 探索两步进针法卵胞浆内单精子注射（ICSI）技术对受精和胚胎发育的影响。方法 回顾性分析2018年1月至2024年5月在江门市中心医院生殖医学中心行ICSI的274个周期的临床资料。根据ICSI操作方法不同分为一步进针法组（OSP组，69个周期）和两步进针法组（TSP组，205个周期），比较两组患者的受精及胚胎发育情况。结果 两组患者间体质量指数、不孕年限、原发不孕比例、基础窦卵泡数比较均无显著性差异（P>0.05）。两组患者间获卵数、成熟卵率、卵子退化率和D3优质胚胎率比较均无显著性差异（P>0.05）;TSP组的受精率（81.06%vs.75.11%）、正常受精率（77.54%vs.71.69%）、正常卵裂率（97.65%vs.96.02%）、D3可用胚胎率（84.78%vs.80.76%）、可用囊胚率（86.38%vs.77.38%）和优质囊胚率（38.25%vs.28.51%）均显著高于OSP组（P<0.05）。相关性分析显示ICSI受精率与精子浓度、精子总活力、精子正常形态率不存在显著相关性（P>0.05）。结论 TSP-ICSI技术可能有助于改善ICSI受精率和促进胚胎发育，但仍需进一步完善研究设计、开展前瞻性研究加以探讨。
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基本信息:
中图分类号:R714.8
引用信息:
[1]戢开丽,杨莹,黎淑贞.两步进针法卵胞浆内单精子注射对受精和胚胎发育的影响[J].生殖医学杂志,2025,34(10):1398-1403.
2025-10-15
2025-10-15