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目的 分析多囊卵巢综合征(PCOS)患者卵泡液中游离脂肪酸(FFA)的代谢差异，筛选差异FFA标志物，探索相关代谢途径。方法 采用气相色谱-质谱联用(GC-MS)技术对PCOS患者(n=13)和对照组(n=8)的卵泡液样本进行51种FFA的高通量靶向定量检测，根据定量结果利用OriginPro 2022软件和SMPDB代谢物数据库进行代谢组学分析。结果 PCOS组和对照组间卵泡液的FFA代谢具有差异性，共筛选出28种差异FFA。其中有21种FFA在PCOS组中的表达显著上调(P<0.05),包括亚油酸、γ-亚麻酸、α-亚麻酸、花生四烯酸等；7种FFA表达显著下调(P<0.05),包括月桂酸、顺-9-肉豆蔻烯酸、反油酸等。SMPDB数据库富集分析后，筛选出α-亚麻酸和亚油酸代谢、脂肪酸生物合成、花生四烯酸代谢等6条脂肪酸代谢通路。结论 筛选出28种PCOS相关的差异FFA,以及6条脂肪酸代谢通路，可为今后PCOS的预防、治疗提供一定的依据和治疗靶点的参考。
Abstract：Objective:To investigate the metabolic differences of free fatty acids(FFAs) in follicular fluid of patients with polycystic ovary syndrome(PCOS),screen the differential FFAs and explore the related metabolic pathways.Methods:High-throughput targeted quantitative detection of 51 FFAs was performed on follicular fluid samples from PCOS patients(n=13) and control group(n=8) by using gas chromatography-mass spectrometry(GC-MS) technology. The software Originpro 2022 and SMPDB metabolite database were used for metabolomic analysis based on the quantitative results.Results:There were differences in free fatty acid metabolism between PCOS group and control groups. Among 28 FFAs screened, 21 FFAs were significantly up-regulated in PCOS group(P<0.05),including linoleic acid, γ-linolenic acid, α-linolenic acid, arachidonic acid, etc. Seven FFAs were significantly down-regulated in PCOS group(P<0.05),including lauric acid, cis-9-myristoletic acid, elaidic acid, etc. Six fatty acid metabolism pathways, including alpha linolenic acid and linoleic acid metabolism, fatty acid biosynthesis, and arachidonic acid metabolism, were screened out through SMPDB database enrichment analysis.Conclusions:Twenty-eight differential free fatty acids and six fatty acid metabolism pathways are screened in this study, which may provide a certain basis and treatment target reference for the prevention and treatment of PCOS in the future.
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基本信息:
中图分类号:R711.75
引用信息:
[1]程铭,贾婵维,马延敏,等.基于高通量靶向代谢组学筛选PCOS患者卵泡液差异游离脂肪酸[J].生殖医学杂志,2022,31(09):1259-1266.
基金信息:
首都医科大学附属北京妇产医院北京妇幼保健院“学科带头人”计划专项(XKDTR201804)
2022-05-13
2022
2022-06-22
2022-07-22
2022
1
2022-09-15
2022-09-15