| 166 | 1 | 82 |
| 下载次数 | 被引频次 | 阅读次数 |
目的 探究以烟酰胺(NAM)和烟酸(NA)为主要有效成分的特医食品精优能对卵巢早衰(POF)的挽救作用。方法 对3周龄ICR雌鼠进行单次白消安腹腔注射建立POF模型，对POF模型鼠连续灌胃28 d不同浓度的精优能，确定恰当的精优能处理用量(200 mg·kg-1·d-1)。再按照处理不同将3周龄ICR雌鼠进行分组：空白对照组(CTRL组),给予PBS连续灌胃28 d;空白精优能处理组(C+200组),给予200 mg·kg-1·d-1精优能连续灌胃28 d;模型对照组(B组),白消安单次注射后，予PBS连续灌胃28 d;模型鼠精优能处理组(B+200组),白消安单次注射后，予200 mg·kg-1·d-1精优能连续灌胃28 d。各组处理28 d后，统计各组小鼠体重、卵巢指数、子宫指数；利用免疫组织化学染色法统计卵泡总数及各级卵泡比例；染色法采用卵母细胞体外培养技术统计卵子成熟率；并通过实时定量荧光PCR、蛋白质免疫印迹法检测卵巢内卵泡发育相关基因、蛋白及部分抗氧化酶的表达量。结果 持续200 mg·kg-1·d-1的精优能灌胃28 d能够显著改善POF小鼠卵巢部分抗氧化酶(Sod2、Cat、Gpx4)的表达水平(P<0.05),显著改善POF小鼠的卵巢和子宫指数(P<0.05),显著增加卵泡数目(P<0.05),显著改善卵泡发育及卵巢储备相关基因(Gdf9、Bmp15、Amh)的表达水平(P<0.05),以及显著提高GV期卵母细胞数量和第一极体排出率(P<0.05)。结论 精优能能够提高POF小鼠卵巢的抗氧化能力，维护卵巢储备与卵母细胞成熟，缓解POF的发展。
Abstract：Objective:To explore the rescue effect of VigorKing, a special medical food with nicotinamide(NAM) and nicotinic acid(NA) as the main effective components, on the mice with premature ovarian failure(POF).Methods:POF model mice were established by a single intraperitoneal injection busulfan in three-week-old ICR mice. The mice were given continuous intragastric of different concentrations of VigorKing for 28 days to determine the appropriate concentration(200 mg·kg-1·d-1)of VigorKing. Subsequently, the three-week-old ICR female mice were divided into four groups according to different treatments: administration of PBS continuously by gavage for 28 days in the blank control group(CTRL group);administration of 200 mg·kg-1·d-1 VigorKing continuously by gavage for 28 days in the blank VigorKing treatment group(C+200 group);administration of PBS continuously by gavage for 28 days after a single injection of busulfan in model control group(group B);administration of 200 mg·kg-1·d-1 VigorKing continuously by gavage for 28 days after a single injection of busulfan in the model rats in the model VigorKing treatment group(B+200 group). After the treatment for each group, the body weight, ovarian coefficient and uterine coefficient of mice in each group were analyzed. The total number of follicles and the proportion of follicles at different grades were determined by immunohistochemistry. The oocyte maturity rate was tested by oocyte culture in vitro. The expression levels of proteins related to follicle development and antioxidant enzyme genes in ovarian were detected by Western blot and real-time quantitative fluorescent PCR respectively.Results:Continuous gavage of 200 mg·kg-1·d-1 VigorKing for 28 days significantly increased the expression levels of some antioxidant enzymes(Sod2,Cat,Gpx4) in the ovary of POF mice(P<0.05),significantly improved the ovarian and uterine indices(P<0.05),significantly increased the number of follicles effectively(P<0.05),and significantly enhanced the expression levels of genes related to follicle development and ovarian reserve(Gdf9,Bmp15,Amh)(P<0.05). The GV stage oocyte numbers and the first polar body extrusion(PBE) rate were also improved(P<0.05).Conclusions:VigorKing can improve ovarian antioxidant capacity of mice with POF,maintain ovarian reserve and oocyte maturation, and relieve the development of POF.
[1] Wang JJ,Tan SJ,Song Y,et al.Improvement of ovarian insufficiency from alginate oligosaccharide in mice[J].J Funct Foods,2022,91:104995.
[2] He Y,Chen Q,Dai J,et al.Single-cell RNA-Seq reveals a highly coordinated transcriptional program in mouse germ cells during primordial follicle formation[J].Aging Cell,2021,20:e13424.
[3] 王丽媛，甄秀梅，王丽娜，等.卵巢储备功能减退患者辅助生殖技术前预处理的研究进展[J].生殖医学杂志，2022,31:1601-1606.
[4] 许梦婷，李芳，王莉莉.卵巢功能早衰的病因与治疗研究进展[J].中国预防医学杂志，2022,23:394-400.
[5] Feng YQ,Wang JJ,Li MH,et al.Impaired primordial follicle assembly in offspring ovaries from zearalenone-exposed mothers involves reduced mitochondrial activity and altered epigenetics in oocytes[J].Cell Mol Life Sci,2022,79:258.
[6] Li MH,Wang JJ,Feng YQ,et al.H3K4me3 as a target of di(2-ethylhexyl) phthalate(DEHP) impairing primordial follicle assembly[J].Chemosphere,2023,310:136811.
[7] Wang JJ,Yu XW,Wu RY,et al.Starvation during pregnancy impairs fetal oogenesis and folliculogenesis in offspring in the mouse[J].Cell Death Dis,2018,9:452.
[8] Jiang Y,Zhang Z,Cha L,et al.Resveratrol plays a protective role against premature ovarian failure and prompts female germline stem cell survival[J].Int J Mol Sci,2019,20:3605.
[9] Tang DY,Feng XS,Ling L,et al.Experimental study for the establishment of a chemotherapy-induced ovarian insufficiency model in rats by using cyclophosphamide combined with busulfan[J].Regul Toxicol Pharmacol,2021,122:104915.
[10] Verma P,Sharma AK,Shankar H,et al.Role of trace elements,oxidative stress and immune system:a triad in premature ovarian failure[J].Biol Trace Elem Res,2018,184:325-333.
[11] Kordowitzki P,Ho WJ,Listijono DR.Nicotinamide adenine nucleotide-the fountain of youth to prevent oocyte aging?[J].Cells,2021,10:2441.
[12] Yang Q,Cong L,Wang Y,et al.Increasing ovarian NAD+ levels improve mitochondrial functions and reverse ovarian aging[J].Free Radic Biol Med,2020,156:1-10.
[13] Miao Y,Cui Z,Gao Q,et al.Nicotinamide mononucleotide supplementation reverses the declining quality of maternally aged oocytes[J].Cell Rep,2020,32:107987.
[14] Bertoldo MJ,Listijono DR,Ho WJ,et al.NAD+ repletion rescues female fertility during reproductive aging[J].Cell Rep,2020,30:1670-1681.
[15] Wang H,Zhu S,Wu X,et al.NAMPT reduction-induced NAD+ insufficiency contributes to the compromised oocyte quality from obese mice[J].Aging Cell,2021,20:e13496.
[16] Min H,Lee M,Cho KS,et al.Nicotinamide supplementation improves oocyte quality and offspring development by modulating mitochondrial function in an aged caenorhabditis elegans model[J].Antioxidants(Basel),2021,10:519.
[17] Wang S,Sun M,Yu L,et al.Niacin inhibits apoptosis and rescues premature ovarian failure[J].Cell Physiol Biochem,2018,50:2060-2070.
[18] Li Y,Dong Z,Liu S,et al.Astaxanthin improves the development of the follicles and oocytes through alleviating oxidative stress induced by BPA in cultured follicles[J].Sci Rep,2022,12:7853.
[19] 邹媛婷，张健，王共明，等.生物活性肽在特殊医学用途配方食品中的应用研究进展[J].食品与发酵工业，2022,48:336-344.
[20] 谷旭照，沈豪飞，高敏，等.双酚A对卵巢功能的影响研究进展[J].生殖医学杂志，2022,31:1445-1449.
[21] Zhao Y,Zhang P,Ge W,et al.Alginate oligosaccharides improve germ cell development and testicular microenvironment to rescue busulfan disrupted spermatogenesis[J].Theranostics,2020,10:3308-3324.
[22] Rezaei F,Bayat M,Nazarian H,et al.Photobiomodulation therapy improves spermatogenesis in busulfan-induced infertile mouse[J].Reprod Sci,2021,28:2789-2798.
[23] 陈子江，田秦杰，乔杰，等.早发性卵巢功能不全的临床诊疗中国专家共识[J].中华妇产科杂志，2017,52:577-581.
[24] El-Derany MO,Said RS,El-Demerdash E.Bone marrow-derived mesenchymal stem cells reverse radiotherapy-induced premature ovarian failure:emphasis on signal integration of TGF-beta,Wnt/beta-Catenin and hippo pathways[J].Stem Cell Rev Rep,2021,17:1429-1445.
[25] Lv X,Guan C,Li Y,et al.Effects of single and multiple transplantations of human umbilical cord mesenchymal stem cells on the recovery of ovarian function in the treatment of premature ovarian failure in mice[J].J Ovarian Res,2021,14:119.
[26] 李仲康，郑嘉华，田彦鹏，等.间充质干细胞治疗卵巢早衰的最新进展及机制[J].中国组织工程研究，2022,26:141-147.
[27] Kumar M,Pathak D,Venkatesh S,et al.Chromosomal abnormalities & oxidative stress in women with premature ovarian failure(POF)[J].Indian J Med Res,2012,135:92-97.
[28] Kafi M,Ashrafi M,Azari M,et al.Niacin improves maturation and cryo-tolerance of bovine in vitro matured oocytes:An experimental study[J].Int J Reprod Biomed,2019,17:621-628.
[29] Covarrubias AJ,Perrone R,Grozio A,et al.NAD+ metabolism and its roles in cellular processes during ageing[J].Nat Rev Mol Cell Biol,2021,22:119-141.
[30] Navas LE,Carnero A.NAD+ metabolism,stemness,the immune response,and cancer[J].Signal Transduct Target Ther,2021,6:2.
基本信息:
中图分类号:R711.75
引用信息:
[1]冯艳芹,刘璇,刘晓婷,等.特医食品精优能缓解小鼠卵巢早衰发生的研究[J].生殖医学杂志,2023,32(08):1223-1231.
基金信息:
山东省自然科学基金项目(ZR2021MC191); 青岛市科技惠民专项(21-1-4-ny-7-nsh)
2022-11-29
2022
2023-04-06
2023-07-10
2023
1
2023-08-15
2023-08-15