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目的通过高脂高糖饮食诱导雌性大鼠多囊卵巢综合征(PCOS),探讨其生殖和代谢特征。方法将23天断奶雌性大鼠随机分成两组:对照组接受正常饮食(对照组,n=18),实验组接受高脂高糖饮食(HFHS组,n=18),连续喂养14周。观察两组体重、动情周期和卵巢组织学变化,检测两组空腹血糖、空腹胰岛素、计算胰岛素抵抗指数(HOMA-IR)、血脂水平、性激素水平。结果 HFHS组大鼠体重至第3周起显著高于对照组(P<0.001),但两组卵巢重量无显著差异[(0.041±0.006)g vs.(0.045±0.005)g,P>0.05]。HFHS组动情周期失去规律变化,发情期持续时间占整个发情周期时间的比例显著长大对照组[(0.46±0.06)vs.(0.27±0.03),P<0.001]。HFHS组卵巢组织学检查发现多个囊状扩张卵泡、黄体数量下降。HFHS组空腹胰岛素、HOMA-IR、总胆固醇水平显著高于对照组(P<0.001);发情间期HFHS组LH和E2水平显著低于对照组(P<0.05),睾酮(T)水平显著高于对照组(P<0.001);发情前期HFHS组LH和P水平显著低于对照组(P<0.05),T和E2水平显著高于对照组(P<0.001)。结论高脂高糖饮食诱导雌性大鼠PCOS,并产生代谢紊乱和卵巢改变,与PCOS患者临床观察到的相似。
Abstract：Objective:To establish a polycystic ovary syndrome(PCOS)animal model of female rats induced by high fat and high sugar diet,and the reproductive and metabolic characteristics of the PCOS rats were also explored.Methods:The female rats with 23 days old were randomly divided into two groups:the rats received a normal diet in control group(n=18),and the rats received a high-fat and high sugar diet in HFHS group(n=18),continuous feeding for 14 weeks.The body weight,estrus cycle and histology of ovary in the two groups were observed.The fasting glucose,fasting insulin,Insulin resistance index(HOMA-IR),lipid profile,hormonal levels were also measured.Results:The body weight of HFHS group increased significantly from the third weeks than that of control group(P<0.001).However,there were no significant differences in ovarian weight between two groups[(0.041±0.006)g vs.(0.045±0.005)g,P>0.05].The estrous cycle of HFHS group lost regular changes.The proportion of estrus duration to the time of total estrus cycle in HFHS group(0.46±0.06)was significantly longer than that of the control group(0.27±0.03)(P<0.001).The results of ovarian histological examination showed multiple cystic expansion follicles and decrease of the number of corpus luteum.The levels of fasting insulin,HOMA-IR and total cholesterol in the HFHS group were significantly higher than those in the control group(P<0.001).In diestrus,thelevels of LH and E2 in the HFHS group were significantly lower than those in the control group(P<0.05),and testosterone(T)level was significantly higher than that in the control group(P<0.001).In proestrus,LH and progesterone levels in the HFHS group were significantly lower than the control group(P<0.05),and the levels of T and E2 were significantly higher than the control group(P<0.001).Conclusions:High fat and high sugar diets can induce PCOS in female rats,and produce metabolic disorders and ovarian changes which were similar to those observed in patients with PCOS.
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基本信息:
中图分类号:R711.75
引用信息:
[1]古兰·托合提木拉提,叶尔努尔·吐苏甫汗,马玉兰,等.高脂高糖饮食暴露诱导大鼠多囊卵巢综合征研究[J].生殖医学杂志,2019,28(02):169-173.
基金信息:
新疆自治区自然科学基金(2011211C184)
2019-02-15
2019-02-15